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By E. P. Abraham, G. G. F. Newton (auth.), David Gottlieb, Paul D. Shaw (eds.)

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Nevertheless, our interest centered on the possibility that N-hydroxyglycine is an intermediate in hadacidin synthesis. In view of the high chemical reactivity of hydroxylamino compounds and the rapid in vivo incorporation of formate into hadacidin, it would not be surprising that free N-hYdroxyglycine might have an extremely short half-life in the cell. , 1962). By starting with labeled hadacidin obtained in our initial experiments, N-hydroxyglycine with a specific activity of 30,000 cpm/mg was prepared and compared to glycine in its effectiveness as a hadacidin precursor (Table 2).

2) was determined by DUTCHER (1958). Hydroxyaspergillic and aspergillic acids, if they occur together in media, can be separated by counter-current extraction with ether and 25 % H 2 S0 4 (DUTCHER, 1958), or by their differential solubility in extractions with phosphate buffer and chloroform (MACDONALD, 1962). Aspergillic acid and hydroxyaspergillic acid can be chemically degraded to a cyclic dipeptide of leucine and isoleucine, which in turn can be hydrolyzed to leucine plus isoleucine (DUTCHER, J.

Part VIII. The structure of mycelianamide. J. Chern. Soc. 1956, 3717. BIRCH, A. , and H. SMITH: Ciba Found. Symp. Amino Acids Peptides Antimetab. Activity, p. 247 (1958). , and N. JOLLIFFE: Metabolism of trinitrotoluene (TNT) in vitro. J. Pharmacol. Exptl. Therap. 88, 300 (1946). BURNHAM, B. , and J. B. NEILANDS: Studies on the metabolic function of the ferrichrome compounds. J. BioI. Chern. 236, 554 (1961). CRAMER, J. , J. A. MILLER, and E. C. MILLER: N-hydroxylation: A new metabolic reaction observed in the rat with the carcinogen 2-acetylaminofluorene.

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