By Michael C. Flickinger, Stephen W. Drew
Encyclopedias composed of: The Encyclopedia of Molecular Biology, the Encyclopedia of Bioprocess know-how: Fermentation, Biocatalysis, and Bioseparation, the Encyclopedia of telephone know-how, and the Encyclopedia of moral, criminal, and coverage matters in Biotechnology, supplying large insurance of 4 significant modern issues during this box. DLC: Biochemical engineering--Encyclopedias.
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Chem. Biol. 2001, 5, 130–136. 19 2 Characterization of a (Bio-)catalyst Summary Enzymes are a class of macromolecules with the ability both to bind small molecules and to effect reaction. Stabilizing forces such as hydrophobic effects only slightly dominate destabilizing forces such as Coulombic forces of equal polarity; thus the Gibbs free enthalpy of formation of proteins, ∆Gformation, is only weakly negative. In an enzyme reaction, initially free enzyme E and free substrate S in their respective ground states initially combine reversibly to an enzyme–substrate (ES) complex.
13 14 1 Introduction to Biocatalysis y hydrolases (44%), followed by oxidoreductases (30%), dominate industrial biocatalytic applications, and y average performance data for fine chemicals (not pharmaceuticals) applications are 78% yield, a final product concentration of 108 g L–1, and a volumetric productivity of 372 g (L · d) –1. Performance data for biocatalytic reactions and processes will be discussed throughout this book, especially in Chapters 2, 7, 18, and 19. 3 lists some of the largest-scale biocatalytic processes, categorized by size stated as of tons per year (tpy).
Kula and M. Pohl, Stabilization of NAD-dependent formate dehydrogenase from Candida boidinii by site-directed mutagenesis of cysteine residues, Eur. J. Biochem. 2000, 267, 1280–89. D. I. Stirling, The use of aminotransferases for the production of chiral amino acids and amines, in Chirality in Industry, A. N. Collins, G. N. Sheldrake, and J. ), Wiley, New York, 1992, pp. 209–222. A. J. J. Straathof, S. Panke, and A. Schmid, The production of fine chemicals by biotransformations, Curr. Opin. Biotechnol.