Download PDF by Claudio Ortolani: Flow Cytometry of Hematological Malignancies

By Claudio Ortolani

Flow Cytometry of Hematological Malignancies includes an array of graphical outputs produced by means of the approach within the research of the main (and the least) universal ailments. the pictures integrated let you examine your individual effects with a 3rd social gathering reference trend.   there's a precise description of the most leukocyte antigens, including an outline in their distribution among general and irregular blood cells. The booklet additionally offers a entire description of the phenotype of each neoplastic blood affliction recorded within the WHO class method, together with all of the directions had to realize and classify even the least universal entity.

Designed to be sensible, the e-book is ideal for speedy session and is split into major sections. part I offers with the direct item of immunophenotyping, and part II offers with the last word goal of the research. greater than 50 antigens are lined and each antigen is handled in 3 major components: common gains, cytometric gains and functional tricks.

This authoritative and state of the art reference might be beneficial for clinicians at once keen on the prognosis and research of hematological illnesses, together with hematologists, hematopathologists, oncologists, pathologists and technicians operating in diagnostic laboratories.Content:
Chapter 1 Antigens (pages 1–157):
Chapter 2 ailments (pages 160–300):

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Extra info for Flow Cytometry of Hematological Malignancies

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E. the lipopolysaccharide-binding protein (LBP) [720]. CD14 is expressed brightly on monocytes, while on promonocytes it is missing or is expressed at a lower intensity [721]. A lower expression of CD14 has also been reported on a minor subset of monocytes characterized by a lower expression of CD11b and CD33 [722], higher expression of HLA-DR [722] and CD45 [723], positivity for CD16 [724], and reduced scatter values [723,725]. CD14 is expressed on pleural macrophages [593], alveolar macrophages [726], peritoneal macrophages [726], and Kupffer cells [727].

CD11b expression has also been demonstrated with immunohistochemical techniques on the membrane of the cells of Langerhans cell histiocytosis (LCH) [36]. 23 CD11b analysis carried out on peripheral blood of three normal donors with a MoAb specific for a calcium-dependent epitope. The samples were anticoagulated either with EDTA (A,B,C, upper row ) or heparin (A’,B’,C’, lower row ). The comparison between the samples drawn from the same donor (A and A’, B and B’, and C and C’ respectively) demonstrates reduced CD11b expression in the samples anticoagulated with EDTA.

4. 11. 2) [674], a 150 kD ectoenzyme encoded by a gene situated on chromosome 15 [675]. CD13 is normally expressed on hematopoietic stem cells [676], on the mature and immature elements of the myeloid and monocytic lineages [677,678], on eosinophils [678], basophils [269,586] and a mast cell subset [589]. It has also been reported on CD19ϩ B-cell precursors in fetal liver and bone marrow [53], on epidermis basal cells [679], and on myeloid but not on plasmacytoid dendritic cells [263]. CD13 expression has been reported on synovial fluid T cells from patients with arthritis [680] and on lymphocytes infiltrating renal cell carcinoma [681].

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