Gastrointestinal Microbiology - download pdf or read online

By Arthur C. Ouwehand, Elaine E. Vaughan

This reference offers a finished and present review of each element of gastrointestinal microbiota. Expertly written chapters conceal traditional and molecular thoughts for the learn of differing microbial populations, in addition to the research of microbial task and interplay with host our bodies. Illustrative and up to date, this resource showcases how fluctuations in our nutrition, surroundings, and pharmaceutical consumption impact microbial composition and behaviour.

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Radajewski S, McDonald IR, Murrell JC. Stable-isotope probing of nucleic acids: a window to the function of uncultured microorganisms. Curr Opin Biotechnol 2003; 14:296–302. 123. Boschker HTS, Middelburg JJ. Stable isotope and biomarkers in microbial ecology. FEMS Microbiol Ecol 2002; 40:85–95. 124. Manefield M, Whiteley AS, Griffiths RI, Bailey MJ. RNA stable isotope probing, a novel means of linking microbial community function to phylogeny. Appl Environ Microbiol 2002; 68:5367–5373. 125. Corthier G, Delorme C, Ehrlich SD, Renault P.

29. Nagashima K, Hisada T, Sato M, Mochizuki J. Application of new primer-enzyme combinations to terminal restriction fragment length polymorphism profiling of bacterial populations in human feces. Appl Environ Microbiol 2003; 69:1251–1262. 30. Muyzer G, de Waal E, Uitterlinden A. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 1993; 59:695–700. 31. Rosenbaum V, Riesner D.

A membrane-impermeable nucleic acid dye propidium iodide (PI) was used in combination with the intrinsic scatter parameters of the cells to discriminate fecal cells from large particles. Coupling FCM results and image analysis, the authors showed that most of the particles detected with a large forward scatter value corresponded to aggregates most likely representing mucus fragments and undigested dietary compounds. 0 mm2 (85). The work highlighted the potential of FCM to study anaerobic fecal bacteria without culturing.

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