By R. Romero-González, J. L. Martinez Vidal, A. Garrido Frenich
Commonly mycotoxins are generally made up our minds by means of immunoassay screening equipment or via unmarried compound chromatographic analytical equipment, in accordance with immunoaffinity column cleanup by way of a separation step utilizing skinny layer chromatography (TLC), gasoline chromatography (GC) or liquid chromatography (LC), that have been coupled to standard detectors reminiscent of electron trap detection (ECD), fluorescence or UV-visible detection. in certain cases, specially whilst fluorescence detection was once used, it was once essential to contain a pre- or post-column derivatization step to be able to elevate the detection services of the analytical process. even if, the applying of hyphenated chromatographic thoughts, in particular LC coupled to mass spectrometry (MS) and LC-MS/MS, has numerous benefits together with uncomplicated therapy, as a result of extra freshen up methods with immunoaffinity columns could be kept away from, quick decision and excessive sensitivity.
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8-10 µg/kg  Table 6. 1 mm) UPLC BEH Eluent A: Methanol Eluent B: H2O Eluent A: Methanol/H2O (5 mm ammonium acetate, 20:80) Eluent B: Methanol/H2O (5 mm ammonium acetate, 90:10) Eluent A: H2O (10 mM ammonium acetate) Eluent B: Methanol Eluent A: Methanol (10 mM formic acid) Eluent B: H2O (10 mM formic acid). 03-220 µg/kg  Table 6. 3/Gradient/NI DAD-TOF ESI (positive ionization) 1 pg- 3 ng  Abbreviations: ACN: AcetonitrileAPCI: Atmospheric pressure chemical ionization; APPI: Atmospheric pressure photoionization; DAD: Diode array detection; ESI: Electrospray; IT: Ion trap; NI: Not indicated; Q: Single quadrupole; QqQ: Triple quadrupole; SIM: Selected ion monitoring; TOF: Time of flight.
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